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. 2010 Oct 15;21(20):3506–3517. doi: 10.1091/mbc.E10-03-0259

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 8. — Down-regulation of adducins affects organization of the actin cytoskeleton. (A) Fluorescence labeling shows rapid formation of the circumferential F-actin belt at the intercellular junctions of control SK-CO15 cells during 1 h of calcium repletion (arrows). By contrast, α-adducin–depleted cells demonstrate diffuse and disorganized perijunctional actin filaments (arrowheads). Bar, 10 μm. (B) Measurements of the lateral width of perijunctional F-actin on cross-sections through cell-cell contacts (indicated by dotted lines on Figure 8A) show significantly less compact F-actin bundles in α-adducin–deficient SK-CO15 cells compared with control cell monolayers. *p < 0.0001 compared with control siRNA-transfected cells (n = 50). (C) Immunoblotting analysis of monomeric (G-) and filamentous (F)-actin fractions demonstrates significant increase in the G/F actin ratio in α- and γ-adducin–depleted cells. *p < 0.05 (n = 3).