Figure 2.

Depletion of NHE8 affects endosome morphology (A) HeLa M-cells were transfected with different siRNA oligos as in Figure 1 and fixed and stained using an antibody against the TfR (green). Nuclei were stained with DAPI (blue). (B) Cells were transfected with either water (mock) or the pool of four NHE8 siRNA oligos at 100 nM (NHE8 siRNA) using a double-transfection protocol and were stained using antibodies to EEA1, LAMP1, or TGN46 (green). Nuclei were stained with DAPI (blue). Scale bars, 15 μm.