Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Oct 15;21(20):3540–3551. doi: 10.1091/mbc.E09-12-1053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

PMC Copyright notice

Figure 7. — NHE8-depletion does not affect the overall lumenal pH of dense MVB. (A) HeLa M-cells were treated with NHE8 siRNA using a double-knockdown protocol. Cells were treated with 30 μM DAMP for 30 min before processing for EM. Immunolabeling was carried out with anti-DNP antibodies followed by protein A–15 nm gold on ultrathin cryosections to label the compartments containing DAMP. (B) Quantitation of the data in A. Bar, 500 nm.