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. 2010 Oct 15;21(20):3567–3577. doi: 10.1091/mbc.E10-06-0482

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 5. — Distribution of GFP-SifA in pexΔ mutants. pexΔ cells selected for expression of plasmids carrying GFP-SifA and DsRed-PTS1 were grown in oleic acid for 17 h and then fixed for direct fluorescence microscopy. PEX5 and PEX7 encode receptors for peroxisomal targeting-signal sequences PTS1 and PTS2, respectively; PEX11 regulates peroxisome proliferation. Note that the diffuse cytoplasmic staining in pex5Δ indicates that no functional peroxisomes were assembled. Overlay shows merged images of the GFP and DsRed channels, and in the bottom two cases, also overlaid with the corresponding DIC images. Bar, 2 μm.