Table 1.
Fluoresence microscopic analyses of peroxisomes in wild-type and mutant cells
| Strain background | Average no. of discrete dots/cella |
|
|---|---|---|
| Vector | pGFP-SifAWT | |
| Without oleic acid | ||
| WT | 5.4 ± 2.5 | 5.5 ± 1.9 |
| With oleic acid | ||
| WT | 7.2 ± 4.6 | 3.9 ± 3.7b |
| pex7Δ | 2.6 ± 2.6 | 1.2 ± 1.8 |
| pex11Δ | 3.2 ± 2.9 | 1.5 ± 1.6 |
| pex25Δ | 3.8 ± 3.6 | 4.0 ± 4.2b |
| rho1-4D | 3.5 ± 4.5 | 2.6 ± 2.2b |
Cells transformed with plasmids pDsRed-PTS1 and pGFP-SifAWT (pDV116) or empty vector (p426TEFpr) were grown in medium with or without oleic acid, then fixed and visualized by direct fluorescence microscopy. In experiments with pGFP-SifA, PTS1-labelled peroxisomes were scored in cells expressing GFP-SifA structures. In all cases, >95% cells have overlapping DsRed-PTS1 and GFP-SifA structures. Results were scored in an average of 100 cells per strain, and each experiment was repeated at least once. WT, wild-type strain.
a Average numbers of dots ± SD, are either dispersed or attached, but distinctly small round peroxisomes labelled with DsRed-PTS1.
b Statistically significant difference (p < 0.005) was observed for the average numbers of dots between vector and pGFP-SifA populations in all cases, except in pex25Δ (p = 0.56) and rho1 (p = 0.18) background.