Table 2.

EM analyses of peroxisomes in wild-type cells

Plasmid	Free and distinct (%)a	Peroxisome countb	Clusters of ≥ two peroxisomes with adherent membranesc (%)	Invaginations and exaggerated membranesc (%)
Vector	64.6	0.30	66.7	6.3
pGFP-SifAWT	38.2	0.07	63.2	62.5

Wild-type cells selected for either pGFP-SifA^WT or vector were grown in oleic acid for 18 h before fixing and processing for EM.

^a Percent of total cells analyzed (162 for vector, and 160 for SifA); values add up to more than 100% because cells may exhibit more than one phenotype.

^b Number of free and distinct peroxisomes counted (437 for vector; 105 for pGFP-SifA) per μm² of total cell area assayed (1454.1 μm² for vector; 1496.7 μm² for GFP-SifA).

^c See Figure 4D for depiction of phenotypes. These peroxisomes are not included in the calculation of peroxisome count.