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. 2010 Oct 15;21(20):3601–3614. doi: 10.1091/mbc.E10-01-0054

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 11. — Effect of Cdk5 phosphorylation of p27^Kip1 Ser10 in neural stem cell differentiation. (A) E13 WT NSCs were expanded in bFGF, transfected with control siRNA and Cdk5 siRNA for 48 h and then deprived of bFGF for 3 d to differentiate as neurons. Cells were plated for ICC analysis using TuJ1 expression as a measure of differentiation. Control cells are in a–d (top) and Cdk5 siRNA-transfected cells in e–h (bottom). (B) A similar sample of transfected cells shows the expression pattern of phospho-Ser10-p27^Kip1 antibody in the control cells (a–c, top) and in Cdk5 siRNA-transfected cells (d–f, bottom). Scale bar, 20 μm.