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. 2010 Oct 15;21(20):3601–3614. doi: 10.1091/mbc.E10-01-0054

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© 2010 by The American Society for Cell Biology

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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Figure 12. — Phosphorylation of Ser10 of p27^Kip1 is involved in neurite outgrowth. (A) Cdk5^+/+ NSCs were expanded in bFGF for 3 d and then transfected with Myc-tagged vectors bearing WT p27^Kip1 (a–c), phospho-mimicking p27/10D (d–f) and nonphosphorylatable mutant the p27/10A (g–i). After removal of bFGF for 3 d to induce neuronal differentiation, cells were fixed and prepared for ICC, and immunoprobed with antibody to TuJ1 and Myc. Scale bar, 20 μm. (B) The histogram represents % of cells with well-developed neurites as opposed to cells with blunted neurites or no neurites, as obtained from four independent experiments.