Table 1. Quantification of GFP immuno-positive zebrafish embryos after reporter constructs injection at one-cell stage.

CONSTRUCT	GFP+ [nt/fp] (% of total/of GFP+)	GFP-positive [ectopic] (% of total/of GFP+)	GFP-positive [nt/fp & ectopic]	GFP-negative (including malformed embryos)	total number of injected embryos (n)
0.8Shh:GFP:LfHhb-I2	51 (10.1/32.9%)	104 (20.6/67.1%)	155 (30.6/−%)	350 (69.3%)	505
0.8Shh:GFP:LfHhb-C1	7 (3.7/15.6%)	38 (20.3/84.4%)	45 (24.0/−%)	142 (75.9%)	187
0.8Shh:GFP* (*negative control)	0 (0/0%)	10 (100/8.1%)	10 (8.1/−%)	123 (92.5%)	133

24–26hpf embryos injected with the indicated DNA construct were scored. nt:notochord; fp:floor plate.