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. 2010 Oct 13;5(10):e13332. doi: 10.1371/journal.pone.0013332

Table 1. Quantification of GFP immuno-positive zebrafish embryos after reporter constructs injection at one-cell stage.

CONSTRUCT GFP+ [nt/fp] (% of total/of GFP+) GFP-positive [ectopic] (% of total/of GFP+) GFP-positive [nt/fp & ectopic] GFP-negative (including malformed embryos) total number of injected embryos (n)
0.8Shh:GFP:LfHhb-I2 51 (10.1/32.9%) 104 (20.6/67.1%) 155 (30.6/−%) 350 (69.3%) 505
0.8Shh:GFP:LfHhb-C1 7 (3.7/15.6%) 38 (20.3/84.4%) 45 (24.0/−%) 142 (75.9%) 187
0.8Shh:GFP* (*negative control) 0 (0/0%) 10 (100/8.1%) 10 (8.1/−%) 123 (92.5%) 133

24–26hpf embryos injected with the indicated DNA construct were scored. nt:notochord; fp:floor plate.