Table 2. List of genes selected for Anopheles gambiae mosquito age grading.

Gene/Probe ID	qRT-PCR primerForward primer (5′-3′)Reverse primer (5′-3′)	Size	Age effect*	Blood feeding effect†	Functional assignment	Fold changes‡	Correlation coefficient¶
AGAP009551	TGCCGATTAAGATTCCCAAC TTCAAGGAGTTCCTGCTCGT	83bp	↑	no	Sulfotransferase domain	4.2	0.89
AGAP011615	GTCTTCCAACCGGAGGTACA CTCCTCTGGACCGGATGTTA	81bp	↑	no	Chitin binding Peritrophin-A domain	3.28	0.61
AGAP002827	ATTGGAGCGTACGCGGTTA CCAGCTAGCAGCAATCCGTA	83bp	↑	no	Synaptic vesicle membrane	3.49	0.50
AGAP005501	ACGTGATCGACACGAATCTG GCACACCACTGTTACCGATG	285bp	↓	no	Rossmann-fold NAD(P)(+)-binding proteins	−3.95	0.65
ENSANGT00000015027	GTCATCTTCTCGCGGTTAGC CAGTCCAAGCGTGGTATCCT	185bp	↓	no	Actin activities	−5.37	0.93
AGAP009790	TCCTAGTGCTCGTCAGTGTGA GTTCGCCGGTTTAGCTCATA	82bp	↓	no	Chitin binding Peritrophin-A domain	−4.66	0.52
AGAP007963	GATGCCACCCTTCTTGTTGT AATTCAAACAGGCCGTCAAG	239bp	↓	no	EF-hand, calcium binding motif	−4.33	0.66

*Age effect: “↑” and “↓” indicate gene expression monotonically increases decreases with age.

^†Blood feeding effect: “no” stands for no significant effect of blood feeding on gene expression.

^‡Fold changes: variation in transcript accumulation between 1-day and 28-day old female mosquitoes, regardless of blood their feeding diet calculated from microarray data.

¶Correlation coefficient: Spearman correlation between gene expression values and mosquito ages in laboratory colonized mosquitoes.