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. 2010 Oct 13;5(10):e13344. doi: 10.1371/journal.pone.0013344

Figure 2. Functional knockdown of FOXA2 in the human colon carcinoma cell line Caco-2.

Figure 2

A. Images of fluorescent microscopy of Caco-2 cells transfected with different FOXA2 siRNA probes and empty vector control. Note, the transfection efficiency was similar amongst the three different siRNA probes while functional knock down of FOXA2 was best with one probe termed FOXA2HSS142473 (details are given by the manufacture, Invitrogen, Paisley, Great Britain); G01, G03 and G05 refer to three independent individual experiments; B. FOXA2 and HNF6 gene expression after functional knockdown of FOXA2 in three independent experiments, *p<0.05 when compared to untreated Caco-2 cells; C. Gene expression of HNF6 and FOXA2 in untreated Caco-2 cells of n = 3 independent experiments, *p<0.05. HNF6 gene expression is significantly less when compared to FOXA2 gene expression.