Fig. 5.

LMO4 interacts with HDAC2. a Co-immunoprecipitation of Gal4-HDAC2 full length (FL) and Gal4-HDAC2 286-489 but not Gal4-HDAC2 1-285 with Flag-LMO4. b Co-immunoprecipitation of Gal4-HDAC2 with wild-type LMO4 and LMO4 with mutated second LIM domain (C87S). Mutation of the first LIM domain (C23S) reduced the interaction with HDAC2. n = 3 experiments. c Functional protein interaction revealed by a mammalian two-hybrid assay. Gal4-dependent pG5-luciferase reporter is activated by co-expression of Gal4HDAC2 286 (containing amino acids 286–489) and ActLMO4, demonstrating functional protein interaction. EV Empty vector. n = 8 experiments