Fig. 2.
Morphological changes of Src-transformed cells that are surrounded by normal epithelial cells. (A,B) Immunofluorescence images of ts-Src MDCK cells (stained with CMFDA, green) that are surrounded by normal MDCK cells (A) or by ts-Src MDCK cells (B). Cells were stained with phalloidin (red) and Hoechst 33342 (blue) after 16 hours of the temperature shift. Scale bars; 10 μm. (C) Quantification of cell height. Data are mean ± s.d. *P<5×10−23, **P<2×10−24; n=46, 48 and 53 cells from three independent experiments. Values are expressed as a ratio relative to Src cells alone.