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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Mol Cell. 2005 May 27;18(5):577–587. doi: 10.1016/j.molcel.2005.04.015

Figure 7. ATF2 Is Affecting the Recruitment of the Mre11 Complex to DSB Repair Foci.

Figure 7

(A) Inhibition of ATF2 reduces recruitment of Mre11 to IRIF. MeWo cells infected with ATF2 RNAi were subjected to IR (5 Gy) and 1 hr later, fixed for IHC. Shown is staining with antibodies to Mre11 and γ-H2AX.

(B) Quantification of inhibition of Mre11 recruitment upon inhibition of ATF2. Data shown in panel (A) were quantified by multiple counts and plotted.

(C) ATF2 does not alter expression of Mre11. IMR90 cells were infected with either control or ATF2 RNAi, and 72 hr later, cells were subjected to IR (6 Gy). Proteins prepared 1 hr after IR were analyzed for Mre11 levels (upper panel) and ATF2 expression (middle panel). β-actin was used as a loading control (lower panel).