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. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: J Biol Chem. 2006 Sep 25;281(46):35316–35326. doi: 10.1074/jbc.M606568200

FIGURE 7. Subcellular localization of Siah2 phospho-mutants.

FIGURE 7

A, HeLa cells were transfected with Siah2-HA, Siah2 T24AS29A-HA, and Siah2 T24ES29D-HA. Cells were fixed and subjected to immunofluorescence with anti-HA antibody. B and C, HeLa cells were transfected with Siah2-HA (panel B) and Siah2 T24ES29D-HA (panel C). Cells were treated with nuclear export inhibitor, leptomycin B (100 nm), for 3 h as indicated. Cells were fixed and subjected to immunofluorescence with anti-HA antibody. D, cells were transfected with the indicated HA-Siah2 WT and mutant forms and FLAG PHD3 and processed for immunostaining using antibodies to HA (green) or FLAG (red). Representative pictures are shown for each antibody as well as their overlay. E, cells were transfected with Siah2-HA, SiahT24S29A-HA, Siah2 T24ES29D-HA, and FLAG PHD3 and treated with 20 µm lactacystin for 4 h. Cells were then processed for immunostaining using antibodies to HA (green) or FLAG (red). Representative pictures are shown for each antibody as well as their overlay.

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