Fig 6. Ectopically CIB1-overexpressing cells showed reduced Plk3 activity.
(A) Western blot analysis of cell lysates from mock- and CIB1-overexpressing T47D cells using anti-CIB1 (top panel). Blot was reprobed using anti-a-tubulin antibody to ensure equal loading (bottom panel). (B) Densitometric quantitation of A. (C) Immunoprecipitate kinase assay of Plk3 from mock- and CIB1-overexpressing T47D cell lysates (top panel). The blot was reprobed for Plk3 to ensure equal loading (bottom panel). Shown is a representative blot of three independent experiments.