Table 2.
The proportions of satellite cells stained by NCAM, Pax7, MyoD, myogenin and Dlk1
| Group | Subject | NCAM, Pax7, laminin and DAPI | Pax7, Dlk1, laminin and DAPI | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Number of SCs | % NCAM+/Pax7+ of total SCs | % NCAM+/Pax7− of total SCs | % NCAM−/Pax7+ of total SCs | % MyoD+ of total SCs | % Myogenin+ of total SCs | Number of Pax7+SCs | % Dlk1+ of total Pax7+SCs | ||
| C | 1 | 44 | 95.5 | 4.5 | 0 | Na | Na | 63 | 90.5 |
| 4 | 73 | 95.9 | 2.7 | 1.4 | 0.89 | 0.76 | 135 | 54.8 | |
| 5 | 92 | 88 | 8.7 | 3.3 | 0 | 0 | 72 | 44.4 | |
| 6 | 97 | 94.8 | 3.1 | 2.1 | 0 | 0 | 172 | 76.0 | |
| 7 | 139 | 95.0 | 4.3 | 0.7 | 0 | 0.36 | 248 | 88.3 | |
| 8 | 88 | 92 | 5.7 | 2.3 | 1.84 | 1.19 | 212 | 76.9 | |
| Mean ± SD | 76 ± 35 | 94.1 ± 3.6 | 4.3 ± 2.5 | 1.6 ± 1.2 | 0.5 ± 0.8 | 0.5 ± 0.5 | 150 ± 75 | 71.8 ± 18.5 | |
| P | 2 | 62 | 87.1 | 1.6 | 11.3 | 0.52 | 0 | 76 | 90.8 |
| 3 | 62 | 98.4 | 1.6 | 0 | 0.46 | 0 | 109 | 72.5 | |
| 4 | 120 | 100 | 0 | 0 | 0 | 0 | 154 | 91.6 | |
| 5 | 83 | 96.4 | 3.6 | 0 | 0.45 | 1.35 | 93 | 89.2 | |
| 9 | 68 | 92.6 | 5.9 | 1.5 | 4.58 | 3.05 | 86 | 80.2 | |
| Mean ± SD | 79 ± 24 | 94.9 ± 5.2 | 2.5 ± 2.3 | 2.6 ± 4.9 | 1.2 ± 1.9 | 0.9 ± 1.4 | 104 ± 31 | 84.9 ± 8.3 | |
| PAS | 1 | 36 | 100 | 0 | 0 | Na | Na | 59 | 25.4 |
| 2 | 79 | 91.2 | 8.9 | 0 | 1.49 | 0.9 | 130 | 36.9 | |
| 3 | 161 | 91.3 | 8.7 | 0 | 0 | 0 | 106 | 76.4 | |
| 5 | 97 | 95.9 | 4.1 | 0 | 0.6 | 0 | 139 | 41.7 | |
| 6 | 29 | 96.3 | 3.5 | 0 | 0 | 0 | 108 | 14.8 | |
| 7 | 116 | 92.2 | 6.9 | 0.9 | 0.31 | 0 | 245 | 74.7 | |
| Mean ± SD | 86 ± 50 | 94.5 ± 3.5 | 5.4 ± 3.5 | 0.2 ± 0.4 | 0.5 ± 0.6 | 0.2 ± 0.4 | 131 ± 62 | 45.0 ± 25.0* | |
The abbreviation (*) means significantly different (p = 0.01) from the P group