Fig. 2.

Assessment of HIV-1 specific CD8+ T cell polyfunctionality by multicolor staining for HLA-B*57/5801 and HLA-Cw*07-restricted epitopes. (A) Magnitude of HLA-B*57/5801 and HLA-C restricted epitope responses using multicolor staining, single CD8+ T cell function responses are shown after background subtraction. (B) Comparison of the contribution of individual functions between HLA-B*57/B5801 (○) and HLA-C (●) restricted epitopes in the study subjects (n = 9). The fractions of the response patterns are grouped and color-coded by the number of functions and summarized in pie chart form where each slice of the pie represents the fraction of the total epitope-specific response that consist of CD8+ T cells with the respective number of functions.