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. 2009 Sep 1;60(4):716–728. doi: 10.1111/j.1365-313X.2009.03994.x

Figure 2.

Figure 2

Uracil repair by Arabidopsis cell extracts is dependent on uracil DNA glycosylase (UDG) activity. DNA duplexes containing either U or THF opposite G were incubated with Arabidopsis cell extracts at 30°C for 3 h in a reaction mixture containing either dCTP or all four dNTPs, both in the absence or presence of 2 U of uracil-DNA glycosylase inhibitor (Ugi). Reaction products were digested with HpaII, separated in a 12% denaturing polyacrylamide gel and quantified by fluorescence scanning. The percentage of fully repaired DNA product is shown in the bottom panel.