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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochemistry. 2010 Jun 1;49(21):4440–4449. doi: 10.1021/bi100394j

FIGURE 5.

FIGURE 5

Quantitative analysis of drug resistance in suppressor mutants. Strains were inoculated into 2 ml YPD containing A, clo (1.25–25.0 μM) or B, cyh (0.25–21.6 μM) at an initial cell concentration of 25,000 cells/ml. Cultures were grown in a shaking bath at 30° C for 48 hr prior to determining the cell concentration using A600. Drug-free cultures of each strain served as a control. In each panel ▲ is WT, ■ is S558Y. Because many of the mutant curves superimpose, no attempt was made to distinguish the individual mutants. C, The E244G, S558Y double mutation was made in pSS607 (◆) for comparison with the original chromosomal suppressor mutation (▼).