Fig. 4.
Adhesion of SIgA to cells expressing DC-SIGN. CHO-S control (panels A–C) or DC-SIGN transfected (panels D–F) cells were grown on glass cover slips and treated with FITC-labeled human IgG (A, D), SIgA (B, E), or the lectin RCA-II (C, F) for 30 min at 4 °C. The cover slips were then washed thoroughly to remove unbound ligand, fixed with 4% paraformaldehyde, and visualized by fluorescence microscopy. Scale bar ~100 µm.