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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Immunol Lett. 2010 Mar 31;131(1):59–66. doi: 10.1016/j.imlet.2010.03.005

Fig. 5.

Fig. 5

Co-localization of SIgA and DC-SIGN. DC-SIGN transfected cells were grown on glass cover slips and treated with FITC-labeled human SIgA and anti-human DC-SIGN monoclonal antibody-conjugated to PE for 30 min at 4 °C, as described in Section 2. The cover slips were then washed thoroughly and transferred to 37 °C incubator for 30 min, to allow endocytosis of ligands bound to the cell surface. The cells were then fixed and visualized by confocal laser scanning microscopy. Panels: A, SIgA-FITC; B, Anti-DC-SIGN-PE; (C) Merge of panels A and B. The arrowheads indicate regions in which the distribution of SIgA and DC-SIGN are coincident. Scale bar 20 µm.