TABLE 3.

Suppression of the reduced viability of exonuclease-deficient cells lacking recombination/repair activities

Other chromosomal mutation(s)b	Effect of mutS, rpoC[R919H], and priA300 mutations on colony formation by plasmid-free segregants of pAM401 sbcCD+/ΔsbcCD ΔxonA ΔxseA constructsa
	mut+ rpo+ pri+	mutS	rpoC[R919H]	priA300
None	+	+	+	+
ΔrecG	−		−b	+
recJ284	*	*,c	+	−d
Δrep	−d	*,cd	+
ΔuvrD	*,c	+	+	+
ΔruvABC	*	*,c	*,c	+
ΔruvABC rus-2	+
ΔrecA or recA269	−d		−bd	*,e

^a+, −, and * are defined in Table 1, footnote a.

^bPlasmid-free segregants for pin-prick colonies that cannot be subcultured.

^cThe colonies observed develop outgrowths of suppressors that form large colonies on subculture.

^dPlasmid-free segregants establish robust colonies on 56/2 minimal salts agar where they account for >35% of the total and can be subcultured without difficulty using 56/2 salts media.

^ePlasmid-free segregants can be subcultured on LB agar.