Figure 1.—

Genetic alterations in the TRiC complex affect arsenic susceptibility. (A) As(III)-sensitive heterozygous diploid YKOs identified by genome-wide mutant fitness profiling. Essential genes are colored in red and nonessential genes in black. The network diagram was created with Cytoscape 2.0 (Shannon et al. 2003). (B) Sensitivity of the heterozygous diploid YKOs of all eight TRiC subunits to As(III) at 450 μm. An ho/hoΔ mutant phenotypically indistinguishable from a wild-type yeast served as the control strain. (C) An hoΔ mutant harboring a vector or a plasmid overexpressing a TRiC subunit as indicated was tested for growth on solid SC −Ura that either contained or lacked 1 mm As(III).