Figure 2.—

Arsenic-sensitive haploid YKOs and their genetic relationships with CCT1. (A) As(III)-sensitive haploid deletion mutants identified by genome-wide mutant fitness profiling. A total of 191 were individually verified to be sensitive to As(III) at 400 μm. The number of mutants in each biological process affected and the corresponding percentage among all mutations identified were listed. This plot was derived from Table S2. (B) Growth of representative arsenic-sensitive haploid YKOs of indicated genotypes on a solid synthetic complete (SC) medium that either lacked or contained As(III) at 40 μm, 150 μm, and 800 μm. (C) Partial suppression of the arsenic sensitivity by CCT1 overexpression in various mutants.