Figure 2.—

Morphological phenotype of cenh3-1 plants complemented by GFP–CENH3 and GFP–tailswap. (A) GFP–CENH3 complemented plants are indistinguishable from wild type. (B) GFP fluorescence labels kinetochores in interphase and mitotic cells from anther squashes. Three dimensional images were captured by deconvolution microscopy. (C) GFP–tailswap complemented plants have a variable vegetative phenotype. (D) GFP–tailswap complemented plants have short stature, and are infertile. (E) Centromeres in GFP–tailswap complemented plants are visualized by FISH in somatic nuclei using a 180-bp centromere DNA repeat probe. We did not detect somatic aneuploidy after inspecting >500 nuclei.