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. 2010 Oct;186(2):461–471. doi: 10.1534/genetics.110.120337

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Figure 3.— — Kinetochore localization of CENH3/H3.3 chimeras and complementation of cenh3-1 embryonic lethality. CENH3/H3.3 chimeras were tagged with GFP at their N termini and cloned under the control of the endogenous CENH3 promoter. The indicated transgenic proteins were introduced into cenh3-1/CENH3 T0 plants. Transformed T1 plants were assayed for GFP fluorescence at kinetochores. Complementation of cenh3-1 embryonic lethality (i.e., viability) was assayed by counting seed lethality in siliques from at least eight cenh3-1/CENH3 plants in the T1 generation. ND, not determined; +++, strong GFP fluorescence at kinetochores; +, weaker GFP fluorescence at kinetochores; -, no GFP fluorescence at kinetochores.