Figure 7.—

B. rapa and Z. mays GFP–CENH3 transgenes do not complement cenh3-1. A. thaliana, B. rapa, and Z. mays GFP–CENH3's were cloned under the control of the A. thaliana CENH3 promoter and introduced into cenh3-1/CENH3 T0 plants. Transformed plants were genotyped, and seed lethality was measured in at least five siliques from individual cenh3-1/CENH3 T1 plants. Each dot represents an independent T1 transformant. Noncomplementation is indicated by 25% lethality, while full complementation is indicated by 6.25% seed lethality (or lower). T2 progeny from B. rapa and Z. mays lines showing the lowest seed lethality were planted and genotyped at the endogenous CENH3 locus. We did not recover homozygotes among 24 T2 plants for each line.