TABLE 1.

Complementation of cenh3-1 by CENH3, GFP–CENH3, and GFP–tailswap transgenes

Transgene	Total no. of plants	CENH3/CENH3	cenh3-1/CENH3	cenh3-1/cenh3-1	P-value
CENH3 (Basta)	32 T1	10	14	8
CENH3 (Hygromycin)	27 T1	9	13	5
Total CENH3	59 T1	19	27	13	0.4396
GFP–CENH3	70 T1	22	45	3	0.0003
GFP–tailswap	33 T1	6	27	0	0.0039
GFP–tailswapa	61 T2	15	38	8	0.1757

Transformed plants born from cenh3-1/CENH3 T0 parents were genotyped at the endogenous CENH3 locus in the T1 generation. CENH3 transgenics were selected using either Basta (pCAMBIA3300) or Hygromycin (pCAMBIA1300). cenh3-1/CENH3 GFP–tailswap/+ plants that yielded cenh3-1 homozygous progeny in the T2 generation were selected on the basis of their lower seed lethality in the T1 generation. P-values were calculated using the χ² distribution, with complementation as the null hypothesis.

^aThese plants were the progeny of T1 cenh3-1/CENH3 GFP–tailswap/+ plants that showed 6–25% seed lethality.