Females mated to males whose SP is unable to be cleaved from sperm retain sperm longer in the seminal receptacles. The number of sperm was counted in the seminal receptacles of females mated to males expressing a modified SP [see text and Peng et al. (2005a,b)] or their controls at 4 days and 10 days ASM. Asterisk indicates a statistically significant difference between groups. Total sperm stored by mates of SP-TGQQ mutant males was comparable to controls at 4 days ASM (SR: t = −1.54, Ncont = 17, NSP-TGQQ = 19, P = 0.065), but we observed a significant difference in the number of sperm stored in the seminal receptacle at 10 days ASM (SR: Wilcoxon: Z = −2.68, Ncont = 10, NSP-TGQQ = 10, P < 0.003).