TABLE 2.
Genome location of mutations detected in the Mut2, 3, and 4 lines
| Amino acid change | Distribution of sequence reads |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chromosome | SGD Position | HP tract | Strain | Gene | Mutation | A | G | T | C | Indel | |
| 1 | 139,349–139,358 | 10 | 4 | del | 1 | 11 | 8 | ||||
| 2 | 275,549–275,557 | 9 | 2 | del | 3 | 3 | |||||
| 2 | 92,271–92,279 | 9 | 3 | del | 1 | 10 | 8 | ||||
| 2 | 92,271–92,279 | 9 | 2 | del | 3 | 0 | |||||
| 2 | 423,462–423,469 | 8 | 3 | ins | 8 | 16 | |||||
| 2 | 662,560–662,569 | 10 | 4 | YBR219C | fs; 103–106 | del | 6 | 5 | |||
| 2 | 653,035–653,045 | 11 | 4 | del | 13 | 9 | |||||
| 3 | 212,451–212,457 | 7C | 3 | YCR048W/ARE1 | fs; 176–178 | del | 15 | 11 | |||
| 3 | 275,289 | NA | 4 | YCR091W/KIN82 | 297; V to I | G to A | 12 | 15 | |||
| 4 | 512,796 | NA | 2 | A to T | 8 | 7 | |||||
| 4 | 814,336 | NA | 2 | G to A | 10 | 6 | |||||
| 4 | 929,182–929,193 | 12 | 3 | del | 1 | 5 | 4 | ||||
| 4 | 963,768 | NA | 3 | YDR252W/BTT1 | 120: G to V | T to G | 12 | 18 | |||
| 4 | 231,908–231,914 | 7 | 4 | del | 12 | 12 | |||||
| 4 | 1,386,657–1,386,664 | 8 | 4 | del | 17 | 11 | |||||
| 4 | 470,576–470,584 | 9 | 4 | del | 19 | 12 | |||||
| 4 | 832,716–832,726 | 11 | 4 | del | 15 | 7 | |||||
| 4 | 50,592–50,603 | 12 | 4 | del | 10 | 9 | |||||
| 1,054,759 | NA | 4 | A to T | 9 | 11 | ||||||
| 5 | 305,972 | NA | 2 | C to A | 5 | 5 | |||||
| 5 | 479,369 | NA | 2 | YER155C/BEM2 | 1159; S to C | T to A | 8 | 3 | |||
| 5 | 225,319–225,327 | 9 | 3 | Del | 9 | 7 | |||||
| 5 | 403,576 | NA | 3 | YER122C/GLO3 | 258; A to E | G to T | 8 | 14 | |||
| 5 | 34,325–34,333 | 9 | 4 | del | 11 | 7 | |||||
| 5 | 402,832–402,843 | 12 | 4 | del | 13 | 7 | |||||
| 6 | 223,108–223,118 | 11 | 3 | del | 10 | 7 | |||||
| 6 | 114,200–114,210 | 11 | 4 | del | 6 | 7 | |||||
| 6 | 88,832 | NA | 4 | YFL024C/EPL1 | 504; D to E | G to T | 15 | 13 | |||
| 6 | 225,229 | NA | 4 | YFR034C/PHO4 | 240; R to G | G to C | 9 | 8 | |||
| 7 | 194,092–194,098 | 7 | 3 | YGL163C/RAD54 | fs; 771–773 | del | 13 | 13 | |||
| 7 | 878,690–878,701 | 12 | 3 | del | 6 | 4 | |||||
| 7 | 653,363–653,369 | 7 | 4 | del | 14 | 10 | |||||
| 7 | 882,549–882,558 | 10 | 4 | del | 10 | 6 | |||||
| 7 | 20,017–20,027 | 11 | 4 | del | 10 | 5 | |||||
| 7 | 678,172–678,182 | 11 | 4 | del | 11 | 9 | |||||
| 8 | 150,380–150,386 | 7 | 3 | del | 11 | 1 | 12 | ||||
| 8 | 472,612–472,624 | 13 | 3 | del | 10 | 8 | |||||
| 8 | 288,299 | NA | 3 | YHR092C/HXT4 | 172; K to K | C to T | 5 | 6 | 1 | ||
| 8 | 370,253 | NA | 3 | YHR132W-A/IGO2 | 46: Y to F | A to T | 15 | 12 | 1 | 1 | |
| 9 | 270,327 | NA | 2 | YIL046W/MET30 | 560; A to S | G to T | 5 | 4 | |||
| 9 | 375,856 | NA | 3 | YIR010W/DSN1 | 143; M to I | G to A | 7 | 10 | |||
| 9 | 199,995 | NA | 4 | YIL087C/AIM19 | 41; T to I | G to A | 12 | 18 | |||
| 10 | 445,012–445,020 | 9 | 3 | del | 10 | 6 | |||||
| 10 | 131,051–131,059 | 9 | 4 | del | 11 | 15 | |||||
| 10 | 469,684–469,694 | 11 | 4 | del | 19 | 6 | |||||
| 11 | 162,688–162,695 | 8 | 4 | del | 9 | 1 | 7 | ||||
| 11 | 403,466 | NA | 4 | YKL018C-A | 19; S to S | C to T | 13 | 13 | |||
| 12 | 405,712–405,719 | 8 | 2 | YLR131C/ACE2 | fs; 369–371 | del | 4 | 3 | |||
| 12 | 32,320-32,330 | 11 | 3 | del | 9 | 5 | |||||
| 12 | 964,065 | NA | 3 | YLR420W/URA4 | 95: R to H | G to A | 11 | 12 | |||
| 12 | 1,009,007 | NA | 3 | YLR436C/ECM30 | 746; I to V | T to C | 12 | 12 | |||
| 12 | 363,531-363,537 | 7 | 4 | YLR106C/MDN1 | fs; 68–70 | del | 11 | 13 | |||
| 12 | 201,846-201,856 | 11 | 4 | del | 14 | 9 | |||||
| 12 | 1,047,741 | NA | 4 | YLR454W/FMP27 | 1249: D to G | A to G | 10 | 12 | 1 | ||
| 13 | 763,010-763,016 | 7 | 2 | SNR86 (small nucleolar RNA) | ins | 5 | 5 | ||||
| 13 | 241,855-241,867 | 13 | 3 | del | 12 | 6 | |||||
| 13 | 311,843 | NA | 3 | C to T | 15 | 25 | |||||
| 13 | 139,705-139,709 | 5 | 4 | YML067C/ERV41 | fs; 138–139 | del | 15 | 13 | |||
| 13 | 816,457-816,463 | 7 | 4 | YMR275C/BUL1 | fs; 706–708 | del | 11 | 9 | |||
| 14 | 761,792 | NA | 2 | YNR069C/BSC5 | 267; V to V | C to T | 8 | 6 | 1 | ||
| 14 | 222,733 | NA | 3 | YNL225C/CMN67 | 580; V to M | C to T | 1 | 17 | 10 | ||
| 14 | 435,595-435,601 | 7 | 4 | YNL101W/AVT4 | fs; 199–201 | del | 9 | 10 | |||
| 14 | 481,123-481,129 | 7 | 4 | del | 15 | 8 | |||||
| 14 | 685,574-685,582 | 9 | 4 | del | 12 | 1 | 9 | ||||
| 14 | 575,616-575,626 | 11 | 4 | del | 12 | 8 | |||||
| 14 | 400,002 | NA | 4 | YNL121C/TOM70 | 180; G to STOP | C to A | 15 | 15 | |||
| 14 | 734,521 | NA | 4 | YNR058W/BIO3 | 77; L to L | A to G | 11 | 10 | |||
| 15 | 854,146-854,153 | 8 | 2 | del | 4 | 5 | |||||
| 15 | 874,052-874,057 | 6 | 3 | YOR296W | fs; 1284–1286 | del | 10 | 7 | |||
| 15 | 767,667-767,673 | 7 | 3 | YOR228C | fs; 36–38 | del | 10 | 7 | |||
| 15 | 822,829-822,835 | 7 | 3 | YOR267C/HRK1 | fs; 678–680 | del | 11 | 8 | |||
| 16 | 146,421-146,427 | 7 | 2 | YPL216W | fs; 868–870 | del | 7 | 5 | |||
| 16 | 22,677 | NA | 4 | C to T | 20 | 14 | |||||
| 16 | 131,583 | NA | 4 | YPL222W/FMP40 | 475; A to T | G to A | 11 | 15 | 1 | ||
| 16 | 509,632 | NA | 4 | YPL022W/RAD1 | 980; A to S | G to T | 19 | 18 | 1 | ||
| 16 | 570,131 | NA | 4 | YPR007C/REC8 | 415; S to M | G to A | 11 | 11 | |||
The type of mutation [base substitution, single-nt insertion (ins), single-nt deletion (del)] is shown, as well as the length of the HP tract that contains an indel. The specific Mut line (2, 3, or 4) is indicated under “strain.” All HP tracts were poly(A) or poly(T) except for the mutation in chromosome 3 at 212,451–212,457, which involved a poly(C) tract. For mutations that occurred within an open reading frame, both the gene name and predicted amino acid changes (fs, frameshift) are provided. NA, not applicable. Coordinates are presented as shown in the SGD (http://www.yeastgenome.org/). The number and distribution of the sequence reads are presented for each mutation. The frameshift mutation in YLR106C/MDN1 conferred a recessive lethal phenotype (data not shown).