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Suggested composition of artificial tubular fluid (in mM)
| early proximal | late proximal | distal | |
|---|---|---|---|
| NaCl | 113 | 130 | 45 |
| NaHCO3 | 25 | 10 | 10 |
| KCl | 4 | 4 | 3 |
| CaCl2 | 2 | 2 | 1 |
| Na2HPO4 | 1 | - | - |
| MgSO4 | 1 | - | - |
| glucose | 5 | - | - |
| amino acid mix | 4 | ||
| urea | 5 | 7.5 | 10 |
| pH | 7.4 | 7.4 | 7.0 |
FD&C green No. 3 is added at 0.05% to stain the solution.
