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. Author manuscript; available in PMC: 2010 Oct 14.
Published in final edited form as: J Orthop Res. 2007 Sep;25(9):1128–1135. doi: 10.1002/jor.20400

Figure 1.

Figure 1

Tryptic digest of the Cl-VIP standard (25 μg) fractionated on a reverse-phase C18 column using a 5 to 70% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid gradient over a period of 40 min and analyzed by mass spectrometry for the presence of Cl−. (A) HPLC reverse phase separation profile of a tryptic digest of Cl-VIP peptide monitored at 214nm and (B) positive ion-mass spectrum of the 20.68 min HPLC fraction (5–6 μg) of the N-peptide of Cl-VIP. The mass spectrum of the 20.68 min peak showed a strong (M+H) pseudomolecular ion at m/z 1203.5, with an isotope pattern consistent with the presence of Cl.