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. Author manuscript; available in PMC: 2011 Sep 14.
Published in final edited form as: Dev Cell. 2010 Sep 14;19(3):365–376. doi: 10.1016/j.devcel.2010.08.008

Figure 5.

Figure 5

Zyxin accumulation at SF strain sites is not dependent on zyxin’s interaction with α-actinin, but is facilitated by interaction with VASP.

(A) Schematic of wild-type zyxin and zyxin mutated to interfere with α-actinin binding (zyxΔ1-42), or Mena/VASP protein binding (zyx4F>A).

(B ) Mutant zyxin zyxΔ1-42 and zyx4F>A localize to SFs in zyxin (−/ −) cells.

(C) Kymograph of zyxΔ1-42 and actin-mApple expressed in zyxin (−/ −) cell. For merge, zyx 1-42 is green, actin is magenta.

(D) Intensity and distance graph showing zyxΔ1-42 accumulates at SF strain sites.

(E) Kymograph of zyx4F>A and actin-mApple expressed in zyxin (−/ −) cell. For merge, zyx4F>A is green, actin is magenta.

(F) Intensity and distance graph showing zyx4F>A accumulates at SF strain sites.

(G) Zyx4F>A (n=12) accumulation at stress fiber strain sites is slower than wild-type zyxin (n=13) (p<0.0001) or zyxΔ1-42 (n=12) (p=0.0002).

Data are shown as mean+/− SEM