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. 2010 Oct 6;30(40):13367–13372. doi: 10.1523/JNEUROSCI.1934-10.2010

Figure 1.

Figure 1.

High Wnt tone decreases OPC production from neural progenitors, while low Wnt tone enhances production. Application of Wnt3a to neural progenitor cultures for 4 d decreased the number of PDGFRα- (C, 11.34 ± 0.93%, mean percentage of total cells ± SE) and O4- (D, 8.40 ± 0.53%) positive cells and application of Dkk1 antagonist increased the number of OPCs (E, 23.14 ± 1.88%; F, 15.48 ± 1.56%) compared to control cultures (A, 15.06 ± 0.89%; B, 11.38 ± 0.75%). This is graphed in K. This is not a result of proliferation (Ki-67) or apoptosis (activated Caspase3) of OPCs, as the percentage of proliferating OPCs was greatest in the Wnt3a group (3.19 ± 0.65%, control = 1.89 ± 0.46%, Dkk1 = 2.30 ± 0.27%) and the percentage of apoptotic OPCs was greatest in the Dkk1 group and significantly greater than control (Dkk1 = 1.78 ± 0.49%, control = 0.15 ± 0.08% Wnt3a = 0.20 ± 0.09%) (L). Retroviral infection of neural progenitors with DNLef1-mRFP results in a significant increase in PDGFRα-positive cells (I, 23.74 ± 3.26%) and O4-positive cells (J, 20.07 ± 1.99%) by 4 d after infection compared to mRFP control infection (G, 8.18 ± 1.18%; H, 10.12 ± 1.62%). Arrowheads indicate infected OPCs. This is graphed in M. N = 3 for all groups. Neural progenitor cells were cultured from BAT-gal mice. N, 6.32 ± 1.13% of PDGFRα-positive BAT-gal cells (arrowheads) show detectable Wnt signaling (arrows). Scale bars, 10 μm. *p ≤ 0.05 for ANOVA in K and Student's t test in L and M.