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Difference between the order parameters of the ligand-bound states and apo-Gal3C plotted vs residue number: (A−C) backbone H−N order parameters, (D−F) side-chain methyl axis order parameters; (A, D) ΔO2(lac−apo), (B, E) ΔO2(L2−apo), (C, F) ΔO2(L3−apo). Secondary structure elements are indicated at the top of the graph. Residues within 5 Å of the ligand are highlighted by gray bars. Error bars indicate one standard deviation.
