Table 6.
PCR analysis of the fate of SXT/R391-like ICE mobile genetic elements during ATAD treatment.
| Sampling Point | Season | ||||
|---|---|---|---|---|---|
| Winter | Spring | Summer | Autumn | ||
| January | March | July | October | ||
| Inlet | ND1 | D | D | D | |
| Biosolids | Bulk water | ND | ND | ND | ND |
| Particulate matter | ND | ND | ND | ND | |
1
SXT/R391-like ICE’s were detected using PCR and probes (IntFor1 and IntRev1) to the unique ICE integrase gene. (Table 1). Results were scored visually and recorded qualitatively as detected (D) or not (ND). Positive controls or R391 were utilised and samples spiked with R391 to show its removal.