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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 26;400(3):413–418. doi: 10.1016/j.bbrc.2010.08.092

Figure 4. BAG3 lose its cell adhesion activity in PDZGEF2 gene knockdown cells.

Figure 4

(A) PDZGEF2 knockdown cells (shGEF2) or control retrovirus infected cells were used for overexpression with pcDNA3 myc-bag3 construct (pcDNA3bag3) or pcDNA3 vector alone, followed by immunodetection with an anti-PDZGEF2 antibody (upper panel) and anti-BAG3 antibody (middle panel). Tubulin served as a loading control (lower panel). (B) PDZGEF2 gene knockdown or control infected cells were transfected with full length expression vector of BAG3, followed by an adhesion assay using crystal violet. Data represent mean± SE (n=6, *p<0.01).