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. 2018 Jul 12;14(6):321–336. doi: 10.3727/105221610x12717040569820

Figure 5.

Figure 5

Sensitivity and specificity assessments for window size and probe size for expression of mouse aldoA, cyp2d-9, and bcl-xL. Sensitivity [TP/(TP + FN)] was plotted for these three genes as indicated for each of (A), (C), and (E) according to probe length using window sizes 8 (filled circles), 12 (open circles), 16 (filled squares), 20 (open squares), and 24 (Xs) nt for mouse aldoA (A), cyp2d-9 (C), and bcl-xL (E). Queries for mouse genes were: aldoA [GenBank:NM007438], cyp2d-9 [GenBank:NM01006], and bcl-xL [GenBank:NM009743]. Specificity [TN/(TN + FP)] was plotted for these three genes as indicated for each of (B), (D), and (F) according to probe length using window sizes from 8 to 24 nt for aldoA (B), cyp2d-9 (D), and bcl-xL (F). A drop in specificity of 0.00001 corresponded to approximately 50 false-positive ESTs. In brief, TP is defined as an EST hit in dbEST derived from the gene of interest; FN is defined as a gene-specific EST not identified by the algorithm; FP is an EST hit derived from another gene; TN is an EST hit obtained by the algorithm that was not gene specific. Further details can be found in the text.