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. 2010 Aug 11;24(10):2038–2049. doi: 10.1210/me.2010-0234

Figure 1.

Figure 1

GH rapidly induces gene transcription in vivo. Results are shown of time course studies of gene transcription in liver from GH-deficient male rats treated with a single ip injection of recombinant rat GH (1.5 mg/kg) for 0, 30, or 60 min. The graphs depict accumulation of nascent nuclear transcripts for IGF-I, Socs2, Cish, IGFALS, Spi2.1, and IGF-binding protein 1 (IGFBP-1), measured by RT-PCR, and quantified by using genomic DNA to generate standard curves (33). Transcript levels from a negative control GAPDH averaged approximately 0.1 ng (data not shown) and did not change after GH treatment. Results are representative of three independent experiments. Information on the PCR primers may be found in Table 1.