Figure 2.

GH induces recruitment of Stat5b to the promoters of hormone-responsive genes and promotes loss of Bcl6 from a subset of genes. Results are shown of ChIP experiments by qPCR for Stat5b and Bcl6 using hepatic chromatin harvested from GH-deficient male rats treated with a single ip injection of recombinant rat GH (1.5 mg/kg) for 0, 30, or 60 min. Each graph depicts the strength of binding of Stat5b and Bcl6 to the region bounded by the PCR primers (horizontal arrows) on the map above (see Table 2 for primer DNA sequences and locations). The maps also display the proximal promoter and exon 1 (as a box; a bent arrow indicates the transcription start site) of each gene (Socs2, Cish, Igfals, Spi 2.1, Igf1); an X indicates each potential Stat5b-binding site (a smaller x marks imperfect sites). Results depict the mean ± sd of three independent experiments (the lack of an error bar indicates that the sd is too small to be seen). The negative control is from Gapdh exon 3.