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. 2010 Aug 11;24(10):2038–2049. doi: 10.1210/me.2010-0234

Figure 3.

Figure 3

Variable binding of Bcl6 to GH-inducible Stat5b domains within the Igf1 locus. Results are shown of ChIP experiments by qPCR for Stat5b and Bcl6 using hepatic chromatin harvested from GH-deficient male rats treated with a single ip injection of recombinant rat GH (1.5 mg/kg) for 0, 30, or 60 min. A, Schematic of rat Igf1 locus, showing locations of previously documented GH-inducible Stat5b-binding domains. The graphs illustrate results of binding of Stat5b (panel B) or Bcl6 (panel C) to each Stat5b domain as a function of GH treatment and represent the mean ± sd of three independent experiments (the lack of an error bar indicates that the sd is too small to be seen). The negative control is from IGF-I exon 1. The location and DNA sequences of PCR primers may be found in Table 2. avg neg, Average negative.