Table 1.
DNA sequences of oligonucleotide primers for qPCR of nuclear RNA
| Gene | Location (5′ end) | Top strand (5′ to 3′) | Bottom strand (5′ to 3′) | Amplicon (bp) |
|---|---|---|---|---|
| Igf1 | Intron 1 (+697) | ACTTCTATAGGGCTTCCACGCT | CCCGCGTTTATATCCATGCTTCCT | 93 |
| Socs2 | Exon 1 (+63) | TTAACTCTTGCCAAGTCTCGCC | CGGAGGAAAGGCTCATGCTTCA | 78 |
| Cish | Intron 1 (+203) | TGCGGGATTTGAGAGGGCTTGA | TGTGTGTGTGTGTGTGTGTCCT | 87 |
| Igfals | Intron 1 (+595) | CTTAACTCAGGGTGGGCTGGT | ACCCTCCGGTGCCAGAAATTGTTA | 70 |
| Spi 2.1 | Intron 1 (+405) | TTGCGGAATCATGCTGGGTGAA | ATTTGGGATCTAGGCGGGAAGT | 100 |
| Igfbp1 | Exon 1 (+90) | GAAAGTCGTGACTACTGAGCCA | ACAACAGTTAGGAACTCCGGCA | 93 |
| Gapdh | Exon 3 | ACTCTACCCACGGCAAGTTCAA | ATGGGTTTCCCGTTGATGACCA | 73 |