Table 2.

DNA sequences of oligonucleotide primers for ChIP assays

Gene and domain	Location (5′-end)	Top strand (5′ to 3′)	Bottom strand (5′ to 3′)	Amplicon (bp)
Igf1
5′	−10,054	CCAGCCGTTAAACACCAAGAAG	TGCTAGTCCCAGTACAGCATCT	94
Promoter 1	−1,036	AGCAGGTCTGGCTCATTTCCAT	AGATCAGATGCTGCGCTTTCCA	78
Promoter 1	−541	TAGTGTGTGCCTCCCATACTGCTT	TGAGCTCTGGGACAGTCTGAAA	105
Promoter 1	+10	AAACGCCTCTGTGCTCCAGTTT	TCTCTCTCCCTCTTCTGGCAAAGT	104
R2-4	−86,264	TGAATCTCCAGGGATCACACCA	CTCTTGTTGATTTCATCTTTGACCCA	84
R8-9	−73,096	GTGAGTCTGTGTTAGTCAGG	GGTTATGTAAGTCTGATAGAG	93
R13	−62,806	TGCATTGGCTACCAGGAACTCT	GAGACAGTTGGAAGGAAGGATAGA	76
R34-35	+3,740	GGTGAAACCGCTCACCTTGG	TTCTAAGAAGCAGACAGAGG	103
R53-54	+26,694	TGGCACATGCCATTGACCAGAT	AAGGGCAGACCCATCTTTCTGA	102
R57-59	+43,817	GGCCAATTCATTCGGCAACTGT	TCCTTTCTGAGAACTGGAGACCGT	87
R60-61	+49,147	CCCAACTGAGAAGTAGTAGGCT	GAAACAGTCCCGACAACCTATC	105
Socs2
Promoter	−1,155	CCCAAAGTCACAGCTTCAACTAA	ACTATTCTGTTGCATCACTGCTCA	73
Promoter	−650	TGAGAGCACTCTGTGCAGCTTT	CGCCAGCATCCTAGAAGGTCAA	70
Promoter	−140	TCCCTTGAAGCCTGCGCATTA	AACTTTCCAGGAATCCGCCTCA	86
Cish
Promoter	−620	AGTTCTTCGGTTCTCTTAAGCGCC	ACACTGCATGAAGGTGGGTGAT	71
Promoter	−227	CTTGGAAATCTGTCAAAGGTG	GGAAGCCGCATCTTCCTAGA	113
Intron 1	+203	TGCGGGATTTGAGAGGGCTTGA	TGTGTGTGTGTGTGTGTGTCCT	87
Igfals
Promoter	−607	ACCTTCCCAGACCTGCCTATTT	CTGGGTTTCAGGAAGGACAGTT	96
Promoter	−122	CCAGGTGTTCCTAGAAGAGG	TTGTCCAAGGCCCAGTCTCAG	95
Exon 1	+365	AGGGTTAAAGGAGCGAGAGTGT	AGCAAGGGCCGTCTACAATACA	106
Spi 2.1
Promoter	−1,116	AAAGGCTACCACTCTGTCCTCA	AGATCCCAAACTCCTGTAGCCA	92
Promoter	−618	GGCCTGTAAAGAGTTGTGTTGG	TGTTCACCTTACCAATGCCTATCT	74
Promoter	−164	AAGGTGTGCTCAGGAGATCAGT	TCGGACAACCTGTGCTTTGTGT	101
Igfbp1
Promoter	−990	CCCTACAACCTTTGCCTTCTCT	TGTCCAGCCCTTCAGTGTCTTCA	94
Promoter	−401	AGGAGAGGAAACAACTGTGGGT	TTAGACCAAGAAGGCTTCAGGC	83
Exon 1	+46	ACACAAACCCAGCGAGCATTGA	ATAGTAGCGGAAGTGGTGGTCACA	76
Gapdh
Exon 3		ACTCTACCCACGGCAAGTTCAA	ATGGGTTTCCCGTTGATGACCA	73