Figure 5.

PKC-induced p66^shc activation is prevented by sex steroids. A, p66^shc phosphorylation determined by Western blot in UAMS-32 cells pretreated with vehicle (veh), 10⁻⁸ m of each dendrimer control (DC), E₂, DHT, or estradiol dendrimer conjugate (EDC) for 1 h and treated with veh or 100 μm PMA for 1 h. B, Caspase 3 activity in UAMS-32 cells pretreated as in panel A and treated with PMA for 6 h. C, Luciferase activity in cells transfected with the NF-κB-luc reporter construct after pretreatment as in panel A followed by PMA for 24 h. D, PKCβ phosphorylation determined by Western blot in UAMS-32 cells pretreated as in panel A and treated with or without H₂O₂ for 1 h. Bars indicate means ± sd of triplicate determinations. *, P < 0.05 vs. respective veh; †, P < 0.05 vs. equivalent treatment in veh-treated cells.