Table 1. Expression of defense-related genes after treatment with OGs or Flg22 elicitor.
Fold-change values are shown for a list of genes that was compiled from the literature and is not necessarily exhaustive. Genes which are considered to be differentially expressed after OGs or Flg22 treatment are indicated by SOM cluster number 1 to 16. Genes for which P-value and/or fold-change did not meet the criteria for differential expression are indicated by lack of SOM cluster number. Fold-change values are highlighted in gray if the probability for differential expression did not meet the threshold of P-value <= 0.01. Additional data is shown in Table S3.
gray: P > 0.01
| Fold Change | |||||||
|---|---|---|---|---|---|---|---|
| Accession # | Primary Sequence Name | SOM cluster | OGs 1h | Flg22 1h | Flg22 3h | OGs 3h | function, description |
| AT3G49120 | AtPCb | 1 | 2.26694 | 2.48117 | 1.88257 | 1.84105 | peroxidase |
| AT4G39030 | EDS5/SID1 | 1 | 5.92157 | 6.86355 | 9.88399 | 3.01812 | member of MATE-transporter family; involved in salicylic acid accumulation |
| AT5G06860 | PGIP1 | 2 | 3.67549 | 4.46139 | 12.8306 | 4.43967 | polygalacturonase inhibitor |
| AT5G03730 | CTR1 | 2 | 1.91361 | 1.73267 | 2.08311 | 1.30183 | ethylene signal transduction |
| AT4G31500 | CYP83B1 | 2 | 4.56826 | 4.60403 | 10.18424 | 5.52262 | indole glucosinolate biosynthesis |
| AT1G18570 | MYB51 | 2 | 43.57281 | 47.14094 | 23.83079 | 5.253 | transcriptional regulator of indole glucosinolate biosynthesis |
| AT1G22070 | TGA3 | 2 | 2.53582 | 2.32457 | 2.95589 | 1.46407 | bZIP transcription factor; NPR1-interactor |
| AT2G06050 | OPR3 | 2 | 3.14133 | 2.34511 | 4.24631 | 1.87207 | 12-oxophytodienoate reductase; JA biosynthesis |
| AT1G02400 | GA2ox2 | 3 | 22.33817 | 37.8386 | 13.3917 | 1.7515 | gibberellin 2-oxidase |
| AT1G62300 | WRKY6 | 3 | 12.94864 | 28.05542 | 8.40955 | 1.33013 | WRKY family transcription factor |
| AT5G61900 | BON1 | 3 | 11.74284 | 13.86363 | 3.27195 | 1.15485 | calcium-dependent phospholipid binding protein |
| AT5G47910 | RbohD | 3 | 9.95031 | 16.55408 | 5.7651 | 1.30293 | NADPH oxidase subunit |
| AT3G11820 | SYP121 | 4 | 7.54514 | 12.57165 | 6.31592 | 1.25092 | syntaxin |
| AT3G18690 | MKS1 | 4 | 9.06529 | 17.96876 | 9.23703 | 1.1434 | MAP kinase substrate involved in mediating responses to pathogens |
| AT1G80840 | WRKY40 | 4 | 19.46441 | 34.14794 | 18.53884 | 1.19892 | transcription factor |
| AT5G64900 | PROPEP1 | 4 | 2.06041 | 3.79552 | 3.21544 | 1.02739 | precursor of peptide elicitor AtPEP1 |
| AT5G60410 | SIZ1 | 4 | 3.09122 | 3.0601 | 3.11005 | 1.17487 | SUMO E3 ligase |
| AT4G26200 | ACS7 | 4 | 3.85424 | 10.01719 | 14.91722 | −1.04156 | 1-aminocyclopropane-1-carboxylate synthase; ET biosynthesis |
| AT4G24240 | WRKY7 | 4 | 2.23022 | 3.11578 | 3.99211 | −1.23778 | WRKY family transcription factor |
| AT4G23810 | WRKY53 | 4 | 9.93022 | 23.57074 | 23.97354 | 2.08616 | WRKY family transcription factor |
| AT4G01250 | WRKY22 | 4 | 4.77135 | 19.33567 | 11.1379 | −1.59013 | WRKY family transcription factor |
| AT2G38470 | WRKY33 | 4 | 12.14716 | 23.45343 | 11.84095 | 1.7918 | transcription factor |
| AT2G44490 | PEN2 | 4 | 3.52335 | 4.59113 | 4.50709 | 1.20738 | glycosyl hydrolase |
| AT3G15210 | AtERF4 | 5 | 2.89873 | 3.24405 | 2.43203 | 1.2588 | member of the ERF subfamily B-1 of ERF/AP2 transcription factor family; negative regulator of JA responses |
| AT4G12720 | AtNUDT7 | 5 | 6.19609 | 6.68088 | 2.76755 | 1.39054 | ADP-ribose hydrolase |
| AT3G25070 | RIN4 | 6 | 2.43514 | 2.90552 | 3.06378 | −1.13757 | RPM1-interacting protein |
| AT3G23240 | ERF1 | 6 | 3.56051 | 3.1204 | 5.03905 | 1.11826 | ERF/AP2 transcription factor |
| AT1G01480 | ACS2 | 6 | 2.16728 | 11.71313 | 57.7373 | 1.33638 | 1-aminocyclopropane-1-carboxylate synthase 2; Et biosynthesis |
| AT1G74710 | SID2/ICS1 | 6 | 4.7639 | 2.89091 | 13.83174 | 1.17735 | isochorismate synthase 1 |
| AT1G64060 | RbohF/RbohAp108 | 6 | 1.75047 | 1.92914 | 2.08271 | −1.119 | NADPH oxidase subunit |
| AT4G01370 | MPK4 | 6 | 1.38944 | 1.91089 | 2.48578 | 1.15367 | MAP kinase; negative regulator of SA-mediated responses |
| AT1G64280 | NPR1 | 7 | 4.26672 | 6.46474 | 2.34459 | 1.07064 | SA-responsive regulatory protein |
| AT4G14400 | ACD6 | 7 | 7.44501 | 6.06913 | 1.64623 | 1.07562 | ankyrin repeat family protein involved in resistance to Pseudomonas syringae |
| AT5G14930 | SAG101 | 7 | 3.82158 | 6.74213 | 1.87661 | −1.25093 | EDS1-interacting acyl-hydrolase |
| AT3G52430 | PAD4 | 7 | 6.95661 | 6.42546 | 1.92185 | −1.02807 | lipase-like gene important for signaling in resistance |
| AT3G48090 | EDS1 | 7 | 8.48693 | 12.74302 | 2.40806 | −1.37953 | lipase-like gene involved in defense signal transduction |
| AT3G45640 | MPK3 | 7 | 3.09329 | 6.11567 | 1.52645 | −1.0769 | mitogen-activated protein kinase activated by elicitor |
| AT4G31800 | WRKY18 | 7 | 4.2576 | 4.7268 | 1.02768 | 1.9853 | WRKY family transcription factor |
| AT3G26830 | PAD3 | 8 | 3.87985 | 5.83097 | 16.91262 | 11.46721 | phytoalexin biosynthesis |
| AT3G04580 | EIN4 | 8 | 1.43745 | 1.73138 | 2.31096 | 1.34655 | ethylene receptor |
| AT3G04720 | HEL | 8 | 1.26766 | 1.29309 | 1.90025 | 2.01009 | chitin-binding JA- and Et-dependent PR gene (PR-4) |
| AT1G66340 | ETR1 | 8 | 1.41777 | 1.44174 | 2.16947 | 1.18641 | ethylene receptor |
| AT1G80460 | NHO1 | 8 | 1.93873 | 2.12266 | 4.82533 | 1.8605 | glycerol-kinase like; required for general resistance |
| AT2G25000 | WRKY60 | 8 | −1.31783 | −1.30492 | 5.02184 | 1.32141 | WRKY family transcription factor |
| AT2G20610 | SUR1 | 8 | 1.44927 | −1.04203 | 4.20875 | 2.91981 | indole glucosinolate biosynthesis |
| AT2G37040 | PAL1 | 8 | 3.60304 | 4.37631 | 20.76027 | 3.5253 | phenylalanine ammonia-lyase 1 |
| AT2G22330 | CYP79B3 | 8 | −1.24378 | −1.12053 | 3.49629 | 2.63977 | indole glucosinolate biosynthesis |
| AT1G30900 | VSR | 8 | 1.69063 | 2.59053 | 13.11446 | 1.21465 | putative vacuolar sorting receptor |
| AT4G16760 | ACX1 | 8 | 1.26181 | 1.5494 | 3.4364 | 1.7688 | fatty acyl-CoA oxidase; JA biosynthesis |
| AT1G16540 | ABA3 | 8 | 1.27199 | 2.15752 | 2.82747 | 1.56 | molybdenum cofactor sulfurase; ABA biosynthesis |
| AT5G51700 | PBS2 | 8 | 1.20536 | 1.33801 | 2.08552 | 1.21455 | resistance signaling protein with two CHORD domains |
| AT5G50200 | WR3 | 8 | 5.5762 | 10.55243 | 30.79313 | 5.69847 | involved in jasmonic acid-independent wound signal transduction |
| AT5G47120 | ATBI-1 | 8 | 1.62388 | 1.78325 | 4.21141 | 1.71686 | Inhibitor of Bax (Bax induces PCD) |
| AT5G10030 | TGA4 | 8 | 1.00862 | 1.21056 | 2.21116 | 1.32526 | bZIP family transcription factor |
| AT4G39950 | CYP79B2 | 8 | 4.56408 | 6.46831 | 29.52901 | 5.50559 | indole glucosinolate biosynthesis |
| AT4G37770 | ACS8 | 8 | 1.06071 | 2.63265 | 23.33609 | 1.35139 | 1-aminocyclopropane-1-carboxylate synthase; ET biosynthesis |
| AT4G12490 | PI/LTP | 8 | 2.2429 | 2.10325 | 14.3117 | 5.37318 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein |
| AT2G30770 | CYP71A13 | 8 | 1.47981 | 1.73999 | 13.7609 | 2.11465 | |
| AT1G52340 | ABA2 | 10 | −2.07308 | −1.5821 | −1.43974 | −1.04607 | short chain dehydrogenase/reductase; ABA biosynthesis |
| AT1G62960 | ACS10 | 10 | −2.01836 | −2.26089 | −1.25227 | 1.27219 | 1-aminocyclopropane-1-carboxylate synthase, putative / ET biosynthesis |
| AT1G01040 | DCL1 | 10 | −2.3136 | −1.94174 | 1.21598 | 1.2237 | RNA helicase involved in microRNA processing |
| AT5G11270 | OCP3 | 10 | −1.87224 | −2.57857 | −1.88235 | −1.15812 | homeodomain transcription factor |
| AT5G03280 | EIN2 | 10 | −2.13118 | −3.51116 | −1.24617 | 1.05215 | Nramp metal transporter involved in ethylene signal transduction |
| AT2G25490 | EBF1 | 10 | −1.22936 | −2.17572 | −1.155 | −1.13154 | EIN3-binding F-box protein |
| AT1G16410 | CYP79F1 | 12 | −2.57576 | −5.08476 | −3.42404 | −1.32291 | alkylglucosinolate biosynthesis |
| AT5G15410 | DND1 | 12 | −1.85683 | −2.07348 | −3.63508 | 1.06433 | cyclic nucleotide gated chanel 2 |
| AT5G60890 | ATR1 | 12 | −1.59561 | −3.47583 | −5.44613 | −1.00113 | Myb-like transcription factor, regulator of tryptophan biosynthesis |
| AT3G54920 | PMR6 | 12 | −1.75391 | −1.58379 | −4.35119 | −1.39162 | pectate lyase |
| AT5G54250 | CNGC4 | 12 | −7.68833 | −4.51407 | −20.63917 | 1.41286 | cyclic nucleotide gated chanel 4 |
| AT4G03190 | GRH1 | 12 | −2.34 | −2.20 | −2.79 | −1.07 | F-box protein belonging to the TIR1 subfamily |
| AT5G36910 | THI2.2 | 13 | −2.89677 | −3.82119 | −24.6223 | −8.25751 | thionin |
| AT2G43710 | SSI2 | 14 | −1.34746 | −1.37445 | −2.17222 | −1.00519 | stearoyl-ACP desaturase |
| AT3G47450 | AtNOS1 | 14 | −1.33353 | −1.3796 | −4.06797 | −1.24375 | nitric oxide biosynthesis |
| AT1G14410 | ATWHY1 | 15 | −1.3392 | −1.70894 | −2.8074 | −1.06456 | whirly family transcription factor |
| AT5G24780 | VSP1 | 15 | −1.84696 | −2.15162 | −3.22115 | −1.86496 | acid phosphatase similar to soybean vegetative storage protein |
| AT4G13770 | CYP83A1 | 15 | −1.903 | −3.14274 | −6.75177 | −1.66531 | alkylglucosinolate biosynthesis |
| AT5G55450 | LTP inhibitor | 16 | −6.14947 | −10.3333 | −27.18177 | −3.4823 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein |
| AT5G48485 | DIR1 | 16 | −1.19926 | −1.40186 | −2.49838 | −1.41223 | lipid transfer protein involved in systemic acquired resistance |
| Genes for which role In defense has not been confirmed | |||||||
| AT1G26380 | FAD linked oxidase | 1 | 100 | 100 | 100 | 35.29239 | FAD linked oxidase |
| AT3G50770 | CML41 | 1 | 4.25762 | 1.44699 | −1.07078 | 4.93792 | calmodulin-like gene |
| AT1G72520 | LOX4 | 4 | 100 | 100 | 66.07086 | 1.18337 | oxylipin biosynthesis |
| AT5G51060 | RbohC | 4 | 1.77213 | 2.64812 | 2.73401 | 1.05192 | NADPH oxidase subunit |
| AT1G28370 | AtERF11 | 5 | 8.51598 | 9.81073 | 4.50834 | 1.38323 | AP2/ERF-family transcription factor |
| AT3G23230 | TDR1 | 7 | 50.95254 | 96.45851 | 2.17893 | 1.29725 | AP2/ERF-family transcription factor |
| AT1G17420 | LOX3 | 7 | 18.18555 | 28.05238 | 8.63377 | 1.14163 | oxylipin biosynthesis |
| AT5G57220 | CYP81F2 | 7 | 100 | 100 | 100 | 62.80708 | cytochrome P450 |
| AT4G23550 | WRKY29 | not on array | WRKY transcription factor | ||||
| Defense-associated genes that are not regulated by OGs or Flg22 at 1 or 3 hours | |||||||
| AT1G08720 | EDR1 | 1.97349 | 1.88711 | 1.30854 | −1.03968 | MAPKKK; negative regulator of SA-inducible defense responses | |
| AT1G32640 | ATMYC2 | 1.3773 | 1.0251 | 1.04767 | 1.0427 | MYC-related transcription factor; regulator of JA signaling | |
| AT1G54040 | ESR/ESP | −1.2194 | −2.13368 | −3.08859 | −1.22696 | epithiospecifier protein | |
| AT1G72260 | THI2.1 | −1.18052 | −2.0278 | −1.52021 | 1.3135 | cysteine-rich protein with antimicrobial properties | |
| AT1G75040 | PR-5 | −1.15202 | 1.28271 | 1.68832 | −1.02924 | thaumatin-like protein | |
| AT2G13810 | ALD1 | −1.09511 | 1.1212 | 1.17468 | −1.31009 | transaminase | |
| AT2G14610 | PR-1 | −1.09192 | 1.20166 | 1.19592 | −1.05584 | pathogenesis-related gene 1 | |
| AT2G17310 | SON1 | −1.2401 | −1.19719 | −1.08872 | −1.06859 | F-box protein | |
| AT2G26020 | PDF1.2b | 1.86264 | 1.13047 | 1.32386 | 1.2864 | plant defensin | |
| AT2G34690 | ACD11 | 1.0769 | −1.02967 | −1.2127 | 1.01437 | sphingosine transporter | |
| AT2G39940 | COI1 | −1.03987 | −1.45158 | 1.2914 | 1.06913 | protein with leucine-rich repeats and degenerate F-box motif | |
| AT2G40690 | SFD1 | 1.15485 | 1.39039 | −1.32653 | −1.21214 | putative dihydroxyacetone phosphate | |
| AT2G43790 | MPK6 | 1.29986 | 1.45728 | 1.98793 | 1.12297 | map kinase | |
| AT2G44050 | COS1 | −1.18726 | −1.09486 | −1.55499 | −1.18392 | lumazine synthase | |
| AT2G46370 | JAR1 | 1.38052 | 1.02308 | 1.17108 | 1.08501 | acyl adenylase | |
| AT3G06490 | MYB108 | 2.1376 | 2.06449 | 2.40666 | 1.42393 | putative transcription factor | |
| AT3G12250 | TGA6 | 1.00319 | −1.28431 | 1.32729 | 1.04112 | basic leucine zipper transcription factor | |
| AT3G20770 | EIN3 | 1.17287 | −1.00618 | 1.02409 | 1.00753 | transcription factor | |
| AT3G23150 | ETR2 | −1.03984 | −1.73644 | 1.80328 | 1.19964 | ethylene receptor | |
| AT3G51770 | ETO1 | −1.00724 | −1.06728 | 1.31689 | −1.06307 | negative regulator of ACS5 | |
| AT3G56400 | WRKY70 | −1.45035 | −1.08418 | 1.12332 | 1.47573 | WRKY transcription factor; activator of SA-induced genes, repressor of JA-induced genes | |
| AT3G57260 | PR-2 | 1.2002 | 1.28983 | 1.67238 | 1.40779 | beta-1,3-glucanase | |
| AT4G03550 | ATGSL05 | 1.38694 | 1.64238 | 1.61435 | 1.09958 | callose synthase | |
| AT4G11260 | SGT1B | 1.17987 | 1.19443 | 1.54933 | 1.16772 | functions in plant disease resistance signaling | |
| AT4G18470 | SNI1 | 1.16086 | −1.04923 | −1.14191 | 1.31395 | negative regulator of systemic acquired resistance | |
| AT4G20380 | LSD1 | 1.40103 | 1.57547 | −1.01223 | −1.18911 | negatively regulates a plant cell death pathway | |
| AT5G05170 | CESA3 | −1.3179 | −1.21327 | −1.39022 | −1.4155 | cellulose synthase | |
| AT5G06950 | AHBP-1B | −1.21129 | −1.21451 | 1.17007 | 1.08722 | bZIP family transcription factor | |
| AT5G06960 | OBF5 | 1.03036 | −1.04004 | 1.81574 | 1.38075 | basic leucine zipper containing protein | |
| AT5G13160 | PBS1 | 1.25113 | 1.22714 | 1.34789 | −1.17822 | putative serine-threonine kinase | |
| AT5G33340 | CDR1 | 1.2038 | 1.39867 | 1.01585 | −1.36359 | apoplastic aspartic protease | |
| AT5G44420 | PDF1.2 | −1.45824 | 1.17455 | −1.14309 | 1.05224 | plant defensin | |
| AT5G46300 | FLS2 | 1.26391 | 1.96747 | −1.03991 | −1.16088 | LRR receptor-like kinase; flg22 receptor | |
| AT5G51290 | ACD5 | −1.1019 | 1.16199 | 1.4786 | 1.05388 | ceramide kinase | |
| AT5G64930 | CPR5 | 1.0806 | 1.086 | 1.27701 | −1.12058 | regulator of expression of PR genes | |
| AT5G65210 | TGA1 | −1.59837 | −1.5148 | 1.37858 | 1.04294 | bZIP family transcription factor | |