Table 1.

Quantification of neurons that coexpress NADPHd activity and leptin-induced pSTAT3-ir in hypothalamic nuclei

Nuclei	Atlas levels	NADPHd cells	pSTAT3-ir cells	Dual-labeled cells	NADPHd cells expressing pSTAT3-ir (%)	pSTAT3-ir cells expressing NADPHd (%)
MPAl	18	62.8 ± 11.3	43.0 ± 6.3	19.4 ± 3.0	34.0% ± 6.9	46.0% ± 5.5
Arc	31	31.7 ± 1.9	129.3 ± 13.3	9.3 ± 2.0	29.5% ± 5.9	7.2% ± 1.3
DMHv	31	142.3 ± 23.5	127.5 ± 10.4	46.7 ± 5.5	35.0% ± 5.1	36.2% ± 2.0
PH	33	80.7 ± 8.2	39.7 ± 5.0	30.4 ± 3.5	39.6% ± 4.7	78.8% ± 5.4
PMV	33	209.4 ± 8.0	186.8 ± 4.9	136.2 ± 4.4	65.2% ± 1.6	73.0% ± 2.4

We quantified the number of cells that express NADPHd activity, pSTAT3-ir, and both (dual labeled cells). Percentage of NADPHd cells that coexpress pSTAT3-ir and the percentage of pSTAT3-ir cells that coexpress NADPHd activity (n = 3–7 cases/nucleus) was estimated. Data are expressed as mean ± sem. The atlas level designation corresponds to those described by Paxinos and Franklin (47).