Table 1.
Characteristics of Abs used to study signaling mechanisms
| Type | Species | Isotype | λ/κ-chain | NC35a | Epitope |
|---|---|---|---|---|---|
| Neutrals | |||||
| Tab-16 | Hamster | IgG2 | κ | No | 322–341 |
| IC8 | Hamster | IgG2 | κ | No | 322–341 |
| RSR4 | Mouse | IgG2 | λ | No | 322–341 |
| 7G10 | Hamster | IgG2 | λ | No | 337–356 |
| Control | |||||
| Isotype | Mouse | IgG2 | κ | No | No binding |
Fold stimulation was determined by intracellular cAMP assay using CHO cells expressing human TSHR, and blocking was expressed as the percent reduction of cAMP produced by test Abs in the presence of TSH divided by cAMP produced by medium (29, 30, 54). RSR4 Ab was kindly supplied by Dr. B. Rees Smith (RSR Ltd., Cardiff, Wales, UK).
a
NC35 indicates that the Ab binding target was CHO-NC35 cells expressing a TSHR variant lacking residues 316–366 and also included amino acid substitutions at 367–369.