Table 2.
Changes in representative proteins in Ab- and TSH-treated thyroid cells
| PKA
|
PIK/Akt/mTOR/S6K/eIF
|
PKC
|
MAPK
|
||||
|---|---|---|---|---|---|---|---|
| Proteins | N-mAb/TSH | Proteins | N-mAb/TSH | Proteins | N-mAb/TSH | Proteins | N-mAb/TSH |
| Cα/β (T197) | −43/64 | PI3K-δ (110, pan) | 44/2a | α (S657) | 19/−26a | MEK1/2 (S217/221, S4) | 87/−34a |
| Cβ (S338) | −3/29 | PI3K-α (pan) | 23/49 | β1/2 (T500) | 86/−51a | MEK1 (T291) | 29/−46a |
| R2α (S98) | 94/5a | PI3KR4 (pan) | −21/64 | γ (T514) | 44/7a | ERK1/2 (T+Y; T/Y) | 45/−17a |
| R2β (S114) | 80/−16a | PI4K-2β (pan) | 13/43 | γ (T655) | 104/3a | RSK1/2 (S363/369) | 93/−43a |
| PIP5K2α (pan) | 35/27 | γ (T674) | 82/10a | RSK1/3 (T573/577) | 74/−9a | ||
| PDK1 (S244) | 0/51 | δ (Y313) | 79/−23a | MSK1 (S376) | 26/−44a | ||
|
JAK/STATs/SOCS4
|
Akt1 (T308) | 38/76 | δ (T507) | 28/8a | p38α (T180+Y182) | 188/−33a | |
| JAK2 (Y1007/1008) | −58/−31 | Akt1 (S473) | 131/48a | δ (S645) | 28/−13a | Mnk1 (T209/214) | 115/1a |
| STAT1 (Y701) | −9/24 | mTOR (S2448) | 173/−5a | δ (S664) | 112/−46a | JNK (T183/Y185) | −32/−16a |
| STAT2 (Y690) | 180/−10a | S6K-40S (S235) | 104/−29a | η (T655) | 72/−4a | Jun (S73) | −66/−5 |
| STAT3 (S727) | 114/107 | S6K (T229) | 43/−39a | θ (S676) | 31/−19a | Jun (S63) | 61/−15a |
| STAT3 (S705) | −14/43 | elF2Bε (S540) | 45/−1a | ζ/λ (T+T) | 60/46 | Fos (T232) | −18/33 |
| STAT4 (pan) | −23/0 | elF4G (S1107) | −34/27 | ||||
| STAT5A (Y694) | 49/−25a | AMPK-α (T174/172) | −45/−27 |
NF-κB
|
|||
| STAT6 (pan) | 44/−2 | GSK3 (S21/9) | −39/−17 | I-κBα (pan) | −3/17 | ||
| SOCS4 (pan) | −13/−41 | I-κBβ (pan) | 1/0 | ||||
| IKKα (pan) | 69/−33a | ||||||
|
Protein-serine phosphatases
|
ρ/Rac
|
IKKα (S180/181) | −20/−40 | ||||
| PP1/Ca (pan) | 49/4a | ROCK1 (pan) | 188/−8a | IKKβ (pan) | −9/6 | ||
| PP2A/Aα/β (pan) | 123/−4a | ROCK2 (pan) | 249/−28a | IKKγ (NEMO, pan) | 109/−14a | ||
| PP2A/Cα (pan) | 22/−33a | RAC1 (pan) | 38/146 | NF-κB p50 (pan) | 35/30 | ||
| PP2B/Aα(pan) | 31/5a | RAC1 (S71) | 15/36 | NF-κB p65 (pan) | 10/35 | ||
| NF-κB p65 (S276) | 114/−33a | ||||||
A change of 30% increase or decrease over control indicates significant.
a
Significant activation or suppression.