Figure 2.
A, Photomicrographs to illustrate the effect of E2 on migration. In each panel, the vertical line (arrow) represents the starting line for migration, and the right hand side of the panel is the area initially scraped free of cells. T47D cells have been exposed to vehicle or to 1 nm E2 for 24 h. The cells crossing the starting line were counted. B, E2 induced cell migration as a function of dose in T47D cells. The specific step down culture conditions are described in Materials and Methods. Vehicle as well as E2 concentrations from 0.001 to 100 nm are present. C, Effect of the antiestrogen fulvestrant (ICI) at a concentration of 100 nm on the migration of cells stimulated by 10 and 1 nm E2. Results are expressed as mean ± sem. *, P < 0.05 compared with vehicle controls by Student’s t test.